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Image Search Results
Journal: Scientific Reports
Article Title: Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
doi: 10.1038/s41598-020-76255-3
Figure Lengend Snippet: The levels and phosphorylation of AMPK ( A ), S6 ( B ) and mTOR ( C ), and the levels of PGC-1α ( D ) and GLUT4 ( E ) were examined by western blots and quantitated. Results are represented as mean ± SE. Values with different letters means significant differences ( P < 0.05, Tukey’s test).
Article Snippet: The primary antibodies used were phosphor-AMPK (Thr172) (dilution 1:1000, Beyotime Institute of Biotechnology, cat. No. AA393), AMPK (dilution 1:1000, EnoGene Biotech, Cat. No. E1A003B), phospho- S6 (Ser235/236) (dilution 1:4000, CellSignaling Technology Inc., Cat. No. 4858), S6 (dilution 1:2000, CellSignaling Technology Inc., Cat. No. 2217), phospho-mTOR (Ser2448) (dilution 1:1000, CellSignaling Technology Inc., Cat. No. 2971),
Techniques: Phospho-proteomics, Western Blot